特色產(chǎn)品：

綿羊抗大鼠腎小球基底膜血清（PTX-001AGBM）
Sheep Anti Rat Glomerular Basement Membrane (GBM) Serum
--抗GBM型腎炎模型誘導(dǎo)

 

產(chǎn)品名稱	Sheep Anti Rat Glomerular Basement Membrane (GBM) Serum
貨號	PTX-001AGBM
規(guī)格	25ml
保存	-20℃
說明	產(chǎn)品經(jīng)56℃熱處理30min，請避免反復(fù)凍融。解凍后會(huì)有輕微沉淀，注射前3000-5000rmp 離心30min。 注：只做過大鼠炎癥，沒有做過小鼠驗(yàn)證。

應(yīng)用說明：(實(shí)驗(yàn)前請仔細(xì)閱讀實(shí)驗(yàn)流程)

每瓶血清含有足夠的抗體用于誘導(dǎo)至少25只大鼠*（175-200 g體重）被動(dòng)抗GBM腎炎模型。
免疫抗GMB血清：麻醉大鼠，尾靜脈注射抗GMB血清超過15秒，疾病的產(chǎn)生依賴于免疫劑量，依照免疫劑量完全打入體內(nèi)很關(guān)鍵（推薦用量：0.4-0.5ml/100g體重）。由于不同批次抗血清差異，大鼠來源，以及實(shí)驗(yàn)要求的不同，我們建議進(jìn)行劑量-反應(yīng)研究，具體的免疫劑量和疾病的嚴(yán)重程度，需要根據(jù)每個(gè)不同批次血清和產(chǎn)生疾病的嚴(yán)重程度，選擇合適的免疫劑量。

腎病研究：

1）異源性疾病：免疫抗GBM (anti GBM)3-5天后，免疫熒光檢測顯示綿羊IgG在腎小球基底膜內(nèi)呈線狀沉積。大鼠c3也呈線性分布，24小時(shí)后出現(xiàn)蛋白尿。
2）自身疾病：免疫抗GBM抗體8-10天后，檢測大鼠蛋白尿增加，同時(shí)大鼠腎小球IgG(自身抗體) 呈線狀沉積，自體疾病變得明顯。（自體）免疫組化呈線性模式的IgG（圖1）。到3周時(shí)，蛋白尿有望達(dá)到200毫克/24小時(shí)，腎小球病變（圖2）明顯，隨著時(shí)間的推移，嚴(yán)重程度增加，導(dǎo)致腎小球硬化。

圖1.免疫熒光檢測顯示免疫抗GBM3周后腎小球毛細(xì)血管壁大鼠IgG免疫沉積的。

圖2.免疫組化染色顯示免疫抗GBM 3周后新月體形成、系膜細(xì)胞增生、毛細(xì)血管變形。

相關(guān)產(chǎn)品：

貨號	產(chǎn)品名稱	規(guī)格
腎炎模型
PTX-001AGBM	Sheep Anti-Rat GBM Serum	25ml
PTX-002S	Sheep Anti-Rat Fx1A Serum	25ml
PTX-003S	Sheep Anti-Rat Thy-1 Serum	20ml
PTX-000S	Sheep Non-Immune Serum	25ml
組織產(chǎn)品（動(dòng)物疾病模型組織、切片、血漿、尿液）
PTX-001	Anti-GBM (Immune-mediated Glomerulonephritis)	-
PTX-002	Anti-Fx1A (Heymann Nephritis, Membranous Nephritis)	-
PTX-003.	Anti-Thy-1 (Mesangial Proliferative Glomerulonephritis)	-
PTX-004	Diabetic nephropathy (streptozotocin- Type I Diabetes)	-
PTX-005	Control (Normal) Kidney	-
PTX-006	Sheep Kidney Section(anti-GBM and Fx1A membranous nephropathy	-
細(xì)胞
CRGMes-1	Rat Glomerular Mesangial Cells	1x106
CRGEp-1	Rat Glomerular Epithelial Cells	1x106
CBGEn-1	Bovine Glomerular Endothelial Cells	1x106
CMUB-1	Mouse Ureteric Bud Cells	1x106
CMMM-1	Mouse Metanephric Mesynchymal Cells	1x106
CRMM-1	Rat Metanephric Mesynchymal Cells	1x106
抗體
BS-001GBM	FITC- Sheep Anti-Glomerular Basement Membrane IgG	0.5ml
BS-002ECM	FITC- Sheep Anti-Extracellular Matrix (ECM) IgG	0.5ml

參考文獻(xiàn)：

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